Background and objectives: Human papillomavirus (HPV) is the most common sexually transmitted infection globally and is responsible for multiple clinical outcomes, including genital warts and cervical, anal, and oropharyngeal cancers. Despite the well established burden of HPV on both men and women, vaccine uptake among men is critically low in Korea. A preliminary step to combat this issue is to target parents of male adolescents. In the present study, we aimed to assess any change observed in parents’ HPV knowledge and attitudes towards male HPV and subsequent willingness to vaccinate their sons following an HPV educational intervention. Methods: On November 14, 2020, a one-time HPV educational session was conducted and parents (n=136) completed self-administered pre-/post-test questionnaires that included previously validated survey items. Paired t-tests were used to evaluate change in HPV-related knowledge, attitudes and intent for HPV vaccination. Results: Following the intervention, participants reported increased scores in HPV and vaccine related knowledge by approximately 4 and 1.5 points, respectively (p<.001). Parents scored higher on perceived benefits of the vaccine, perceived social influence on vaccination decision and perceived severity of diseases caused by HPV infections, whereas lower scores were observed for perceived harm of the vaccine, and difficulties communicating with their sons about sex and HPV vaccination. Following the intervention, those without the intent to vaccinate their sons significantly decreased and those who intended to vaccinate their sons though not in the next six months significantly increased (χ2=33.045, p<.001) Conclusion: Our educational intervention resulted in an increase in HPV-related knowledge with a positive impact on parents’ attitudes toward male HPV vaccination. We expect that this study can serve as a pivotal reference to promote the unexplored issue of HPV vaccination among male adolescents in Korea.